The present study investigates the phytochemical activity of a few endemic and medicinally important orchids of NorthEast India,
highlighting their potential as valuable sources of bioactive compounds for pharmaceutical, nutraceutical, and industrial applications. The
species exhibited substantial variation in total phenol content (TPC), ranging from 15.70 to 83.16 mg GAE g-¹, with a mean value (43.70 mg
GAE g-¹). Total flavonoid content (6.86-29.54 mg QE g-¹) and flavanol content (0.09-2.35 mg QE g-¹) were similarly diverse, with
Bulbophyllum odoratissimum consistently demonstrating the highest levels across phenolic and flavonoid categories. Antioxidant activity,
measured by FRAP (3.25-16.59 mg FeSO4 g-¹), also varied significantly amongst species, with Liparis odorata exhibiting the greatest
reducing potential. Sugar profiling revealed notable differences in total sugar, reducing sugar, and non-reducing sugars, with sucrose
identified as the predominant carbohydrate in orchid leaves. Correlation analysis indicated a strong positive relationship between total
sugar and reducing sugars (r = 0.998; p < 0.01), while other biochemical parameters showed no significant associations. Overall, the
present findings underscored the biochemical richness and interspecific variability amongst medicinal orchids, suggesting promising
opportunities for further phytochemical characterization, pharmacological evaluation, and potential biotechnological applications.