Aerides multiflora Roxb. is an epiphytic orchid with significant medicinal and ornamental value. The present study was aimed to standardize
mass multiplication through in vitro asymbiotic seed germination on M and MS nutrient media, with and without growth additives. The
investigation focused on evaluating the effects of different combinations of growth regulators on seed germination, protocorm formation
and their subsequent development into seedlings. The highest germination rate (98.75%) was achieved on M medium supplemented with
kinetin (KN) and activated charcoal (AC). Furthermore, M medium enriched with IBA (0.5 mgL-¹) proved suitable for seed germination,
protocorm multiplication, and development of seedlings. The standardized protocol offers an efficient method for the large-scale propagation
of A. multiflora and provides a potential strategy to reduce commercial collection pressures on natural populations of this species.