Mshioe and Skoog (1962, MS ) medium and its different combinations of growth regulators, auxin [|AA, IBA, NAA, 2,4-D (1
6 mol). NAA (1-2 mgl ')l: cytokinin (KN (1-5 mgl')); and gibberellin (GA,(1 mgl')). In the basal medium, explants turned brown
and perished within 50 days. lAA was effective when used at 2-4mgll. At 2 mgl', it invoked regeneration via Protocorm like
bodies PLBs and at 3-4 mgl, it favoured the development of rhizomatous bodies. KN effectively induced regeneration of PLBs in
27 5-66.5% explants when used at concentration ranging from 2-5 mgl:', KN and IAA, when used together, in the ratio of 1:1
1mal each) acted synergistically to induce shoot bud differentiation in 50% explants. Plantlets were transferred to clay pots
containing potting mixture (brick-pieces, pine bark, charcoal, moss) in ratio of 1:1:1:1. Nearly 60% plantlet survival was
recorded.