Present paper reports the regeneration competence of leaf segments in a commercially important orchid, Cymbidium eburneum Lindl., for its mass multiplication. Nearly 83.3% regeneration response could be evoked on M (Mitra et al., 1976) medium supplemented with 6 Benzyladenine (BA, 0.5 mgl-1) and a-Naphthaleneacetic acid (NAA, 2 mgl-1) within 5.25 wks, and 15.7 plantlets per explants could be regenerated after 30 wks of culture initiation. Cytological studies confirmed the uniform somatic chromosome number of 2n=40 in the regenerants. The protocol can be utilized for rapid mass multiplication of this orchid and aid in alleviating the collection pressures on its natural populations.