Environmental adaptations necessitate the modulation of photosynthetic processes and water vapor exchange in plant species, thereby influencing key biochemical and physiological processes that govern overall plant function and productivity. Alpine plant communities are particularly susceptible to these environmental perturbations, requiring intricate adaptive mechanisms to sustain their ecological viability. Given the heightened sensitivity of high-altitude flora to climate-induced shifts, it is crucial to elucidate species-specific responses to these changes. The present study investigates the eco-physiological and biochemical variations in Dactylorhiza hatagirea along an altitudinal gradient, providing insights into the adaptive strategies of high-elevation species under climate warming scenarios.