An efficient and reproducible protocol for asymbiotic seed germination of an endangered and therapeutically important terrestrial orchid Herminium lanceum, commonly known as Jalya, has been established. Its leaves and stem are mainly used for curing fever, diabetes, and urinal disorders. With a view to enlarging its population base, an attempt was presently made to culture its mature seeds procured from dehisced capsules on modified Knudson C (KC, 1946) medium fortified with different concentrations of auxins (IAA, IBA, NAA; 1 mgl-1 each and cytokinins (KN, BAP; 1mgl-1 each) in different combinations. The aim of present investigation was to study the effect of different combinations of growth regulators on seed germination and their subsequent development into seedlings. Seeds showed initial signs of germination within 29±0.88 days of inoculation. The first visible sign of germination was the swelling of embryos followed by apical rupturing of seed coat so as to release the spherules. Within 95±3.05 days of inoculation, the spherules developed into oval, green protocorms with absorbing hair. The complete seedlings were obtained within 149±1.15 days of inoculation. The highest germination percentage (83.02%) and early development of healthy seedlings were observed on medium augmented with combination of IBA and KN.