Shimla hills located at an altitude range 1600-2800 m amsl, are biodiversity rich zones in NorthWestern Himalayas. Terrestrial orchids have special preference for this area because of its vast vegetation ranges, forest cover, and favourable climate. Orchids are generally highly adapted to form specific biological interactions with other organisms; flowers are usually specialized to specific pollinators and all species rely on mycorrhizal symbiosis with specific groups of fungi. They have been used in many parts of the world in traditional healing system as well as in the treatment of a number of diseases since the ancient time. Orchids are considered as indicator species of habitat disturbances. Recently, these plants are listed amongst the most threatened taxa globally due to increasing anthropogenic threats inherent rarity, and specific conservation needs. The most commonly cited threats affecting orchids are deforestation, logging, forest fire road construction, and the expansion of forest plantations and agriculture, and their over-collection for the ornamental, medicinal, food, and trade. The present investigation deals with substratum analysis of some therapeutically significant and/or endangered orchids from Shimla and adjacent hills. It was observed that these plants grow in a variety of habitats, having different soil types with slightly acidic pH and with species specific nutrient requirements. The information obtained after soil analysis will indicate about the nutritional requirements of species, and this information can later be utilized for formulation of species specific nutrient media for their in vitro mass propagation and conservation programmes.