Crepidium acuminatum (D.Don) Szlach., commonly known as Jeevak, is an endangered medicinal orchid of renowned status in Ayurveda The dried pseudobulbs of C. acuminatum are one of the important ingredients of Ashtavarga Drugs used in the preparation of Chyavanprash and also to cure tuberculosis. Presently, an attempt was made to establish in vitro method for rapid regeneration of C. acuminatum using young unopened floral buds with a view to developing an efficient mass propagation protocol for its conservation. For this purpose, young unopened floral buds from immature inflorescence (8 to 10 days old) were excised, surface sterilized in bavistin (0.01 mgl-1), streptomycin (0.01 mgl-1), and mercuric chloride (0.1 mgl-1) and subsequently cultured on M (Mitra et al., 1976) medium supplemented with different growth additives (1 mgl-1). Interestingly, the buds successfully regenerated into shoot buds in M medium containing IAA and IAA+KN in three wks old cultures; the best response was however, observed on medium containing IAA+KN (1 mgl-1 each) in which 8 to 10 pseudobulbous shoots were developed from single floral bud after 5 months of culturing. Pseudobulbous plantlets complete with roots and leaves were obtained in 21 wks old cultures. However, when BAP (1 mgl-1) was added to basal medium, some buds initially showed swelling after 3 wks of culturing and subsequently, opened into as flowers. These data indicate that C. acuminatum can be successfully regenerated using young floral buds and this methodology may be applied to other medicinally important orchids so as to produce a large number of clones in short time.