The present study deals with antimicrobial activity and anticancerous efficacy of leaf extract of Luisia zeylanica Lindl. by using different solvents. Preliminary phytochemical screening revealed the presence of alkaloids, flavonoids, glycosides, phenols, saponins, tannins, steroids, and terpenoids. These extracts were used to study antimicrobial activity against four bacterial and three fungal strains by agar diffusion method. In vitro anticancerous activity was also carried out against two cancer cell lines (MCF-7 and HeLa cell line) by using 3 (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The highest zone of inhibition was observed in ethyl acetate extract against Lactobacillus acidophilus, whereas methanolic extract showed highest activity against fungus, Candida albicans. A significant cancer cell growth inhibition was observed for two extracts with IC50 value ranging between 18.36 μgml-1 and 67.914 μgml-1.