The present study has been conducted in Khokhan Wildlife Sanctuary of Kullu district, Himachal Pradesh. Intensive and extensive surveys were conducted for the collection of orchid species. Rapid sampling for the qualitative and quadrat method for quantitative assessment has been followed. Total 16 species of orchids representing 11 genera were recorded. These species were distributed between 1,500-2,750 m amsl in shady moist, dry, and degraded habitats. The orchid species were analysed for nativity, endemism, indigenous uses, and threat categories. Amongst the species, 07 species were natives and 09 species were non-natives to the Himalayan Region. The species namely Platanthera edgeworthii, Satyrium nepalense, and Habenaria pectinata were Near Endemic and Habenaria intermedia was Endemic to Indian Himalayan Region. Of the total species, 06 species were found in the sites sampled for quantitative assessment of vegetation. Epipactis helleborine was recorded in maximum sites (05 sites), followed by Calanthe tricarinata (03 sites), Habenariaintermedia, Herminium lanceum, and Malaxis muscifera (02 sites, each) and Epipactis latifolia (01 site). Maximum species were found in shady moist habitat (06 spp.), followed by dry (03 spp.), and degraded (01 sp.) habitats. Quantitative assessment of the orchid species revealed their density very low i.e., < 1.0 ind m-2. Some of the species were used to cure sores, eczema, fever, burns, cough, cold, cuts, rheumatism, nervous disorder, female disorder, kidney disorder, urinary problems, dysentery etc. Maximum species (04 spp.) were used as tonic, followed by some to cure fever (03 spp.), eczema, sores, and urinary problems (02 spp., each). Amongst the part used, tuber and arial part (05 spp., each) were utilized maximum. Habenaria intermedia was Endangered, Malaxis muscifera and Platanthera edgeworthii were Vulnerable and Epipactis helleborine, Herminium monorchis, and Satyrium nepalense were Near Threatened and remaining species were under Least Concern category. Frequent monitoring of habitats using quadrat method is suggested to understand the impact of climate change and anthropogenic activities on the population of orchid species.Received: November