Phalaenopsis orchid is one of the most sought after pot plant as well as cut flower in the international market. It is a short stemmed monopodial orchid, producing lateral inflorescences, usually twice a year in the tropical conditions. To retain the flowers for a longer period, presently, inflorescence pruning and its effect on emergensce of new flower spike and flowering behaviour were studied. The spent (flowered) inflorescences were pruned at three different levels, 1st, 2nd, 3rd node, which was compared with that of control, wherein the spent inflorescences were retained as such. Results showed that the number of days taken for emergence of new flower spike were minimum (23.80 days) in plants where the spent inflorescences were pruned at the first node, compared to that at the third node (36.40 days) and second node (66.60 days). The treatments were significantly superior to non-pruned plants (control) as the new inflorescences emerged only after 174.40 days, in the latter case. The number of days taken for the first bud emergence and the first flower opening, showed no significant difference. Though inflorescence length and number of florets were at par amongst the treatments, flowering duration was longer in plants pruned at the first node.