The current study was intended to facilitate ex situ conservation of floriculturally and medicinally important endangered Coelogyne cristata. The cultures were initiated using asymbiotic seed germination technique on a defined Mitra et al., 1976 (M) medium supplemented with plant growth regulators (PGRs) such as [6-benzyladenine (BA - 4.44 μM), furfuryl aminopurine (KN - 4.65 μM), -naphthalene acetic acid (NAA - 5.37μM) and indole, 3-butyric acid (IBA - 4.92 μM)]. The frequency and onset of germination was markedly influenced by the type of growth regulator used in the nutrient medium. Cytokinins proved optimal for germination and led to fastest development of seedlings within 18.08±0.50 wks of culture. Simultaneously, the efficacy of sucrose at different concentrations (5.0, 10.0, 15.0, 20.0, 25.0 and 30.0 gl-1) was also assessed on the germination percentage of seeds. The growth of the seedlings was best on the medium containing 20 gl-1 of sucrose.