Satyrium nepalense D.Don is a terrestrial, multi-purpose orchid with therapeutic, nutritional, and aesthetic benefits as well as aroma. Its
extensive elemental composition which contains triterpenes, alkaloids, flavonoids, and unsaturated sterols, makes it eligible to be classified
as a medicinal orchid. The juice of the plant is used to treat fever as well as cuts and wounds, and the dried tubers are used as a dysentery
preventative. The survival of the species is threatened by natural factors including habitat loss triggered by undulating topography and
landslides sparked by heavy rains. Its multiplication by conventional and micropropagation methods, as well as its restoration into natural
habitats and niche regions will prove to be a significant conservation effort. Mature seeds (20 wap) procured from dehisced capsules
(pods) of S. nepalense were cultured on MS Medium with or without growth hormones [Auxins (Indole-3-Acetic acid, IAA and 1-Napthalene
Acetic acid, NAA; 0.5 mgL-1 each); Cytokinins (6-Benzylaminopurine, BAP, Kinetin, KN, and Thidiazuron, TDZ; 0.5 mgL-1 each); and
activated charcoal (0.2%)]. MS medium fortified with AC+BAP (0.5 mgL-1) proved an optimal nutritional combination during germination and
seedling development. However, the medium supplemented with NAA (0.5 mgL-1)+BAP (1.0 mgL-1) proved beneficial for rapid protocorm
multiplication. The in vitro seed culture protocol established from the present study may be employed for large scale propagation of this
commercially important S. nepalense and other related taxa.