Acampe praemorsa (Roxb.) Blatt. & Mccann is a monopodial epiphytic orchid which possesses eminent medicinal value. In the present study, four different nutrient media namely MS (Murashige and Skoog, 1962), PM (Phytamax; Arditti, 1977), MVW (Modified Vacin and Went, 1949), and KC (Knudson, 1946) were used for in vitro asymbiotic seed germination. PM basal medium proved the best percentage for seed germination (66.67%) followed by MS (58.34%), MVW (41.67%), and KC (33.34%) media. Phytochemical investigation was conducted for plant parts such as leaf, stem, root (procured from in vivo grown plants) and for in vitro raised plantlets, in A. praemorsa. Maximum amount of alkaloid content was found in leaf in Wagner's and Tannie acid reagent; in stem in Dragendroff's and Tannie acid reagent; in root in Tannie acid reagent; and in in vitro raised plantlets in Wagner's reagent. Amongst other secondary metabolites, the highest amount of tannin, flavonoid, steroid, and phenol was found in leaf, whereas, tannin, steroid, terpenoid,and phenol was found in stem; and tannin, flavonoid, steroid, protein, phenol, quinine, and coumarin were found in root. The highest quantity of saponin, flavonoid, and phenol was observed in in vitro raised plantlets