In the present investigation, seeds procured from green capsules of Habenaria digitata Lindi. were cultured on 0.8% (w/v) agar solidified MS medium supplemented with eight different growth additives namely, activated charcoal (AC), banana powder (BP), peptone (P), vanilla powder (VP), amaranth juice (AJ), coconut water (CW), pineapple juice (PJ), and tomato juice (TJ). Maximum percentage (93.34%) of seed germination was found on MS medium fortified with 1 g1·1 activated charcoal,2 g1·1 peptone, and 20% coconut water. The lowest time required for initiation of seed germination (7.21±0.34 wks), induction of protocorms (10.29±0.49 wks), and development of seedlings (25.79±0.60 wks) was achieved on MS medium supplemented with 1 g1-1 AC. After 30 days of culture, the maximum increase in length
(3.91±0.04 cm) and number of roots (4.17±0.31) was observed on MS medium supplemented with IBA (1.0 mgl 1 + NAA (1.0 mgl 1 . The highest (96.00%) greenish shoot primodia like structures (SPSs) were developed within the lowest time (6.22±0.15 wks) in liquid MS medium supplemented with KN (1.0 mgl·1) and NAA (0.6 mgl-1). The healthy seedlings with well-developed roots were transferred to natural environment following successive phases of acclimatization.