The natural populations of many splendid orchids are declining from their natural habitats at an alarming rate and their conservation is becoming a matter of global concern. The present study was intended to facilitate ex situ conservation of Aerides crispa, an epiphytic orchid having tremendous ornamental and medicinal value. Asymbiotic seed germination potential of immature seeds procured from unripe green capsules was assessed on agar-gelled MS Medium with and without various combinations of plant growth regulators [Auxins (IAA, NAA} and Cytokinins (KN, BAP, TDZ}] and activated charcoal (AC; 0.2%}. The onset of germination initiated within 6.25±0.47 days of culturing on MS Medium fortified with IAA+TDZ (0.5 mg1·1 each}. The percentage germination response showed significant differences in majority of combinations used; it was highest (99.50±0.25%} in the medium supplemented with AC. Early protocorm development (23.25±0.47 days} and their rapid multiplication was observed when medium was supplemented with NAA+KN (0.5 mgl 1 each). First leaf and root primordia were developed within 30.05±0.47 and 82.75±1.10 days of culturing, respectively on medium supplemented with IAA+TDZ (0.5 mgl·1 each); seedling development was recorded in 99.00±1.29 days, in the medium containing NAA+TDZ (0.5 mgl 1 each}. MS medium supplemented with IAA+TDZ (0.5 mgl 1each} proved an optimal nutritional combination during germination and seedling development.