DENDROBIUM IS the largest genus of the Orchidaceae family containing more than 1,800 species that are found in diverse habitats throughout much of South, East and SouthEast Asia, including China, Japan, India, Philippines, Indonesia, Australia, New Guinea, Vietnam, and many of the islands of the Pacific. In Bangladesh, 27 species of Dendrobium are distributed throughout the country especially Chittagong, Chittagong Hill Tracts, Cox’s Bazar, greater Sylhet, Gazipur, and Sundarbans forest (Huda, 2007). Dendrobium is the leading genus for phytochemicals content and 100 compounds including 32 alkaloids, 22 phenanthrenes, 15 bibenzyls, 7 sesquiterpenoids, 6 coumarins, and 4 fluorenones have been identified in 42 Dendrobium species (Hossain, 2015). Dendrobium formosum Roxb. ex Lindl. is an epiphytic pseudobulbous orchid found on trunks of large trees; it flowers in the month of May. The large attractive flowers of the species have horticultural importance. The biological status of this species is Vulnerable (VU) due to habitat destruction and mass collection by orchid hunters (Huda, 2008). In Bangladesh, the species was recorded from Sylhet, Chittagong, and Cox’s Bazar district. The present study was therefore, designed to evaluate the in vitro asymbiotic germination potential of its seeds, growth and multiplication of protocorms, differentiation of first leaf and root primordia, and development of seedlings, with a view to developing mass propagation protocol for this species.