Thailand is the origin of about 1,300 tropical orchid species in 178 genera. Deforestation and over-collection of wild Thai orchids for trade has placed these species at a risk of extinction. Therefore, the conservation, as well as sustainable use is urgently needed to conserve these by various means. The genus Paphiopedilum and Dendrobium cruentum are listed in Appendix I of CITES. Presently, various methods of cryopreservation of Thai orchid species were implemented. For cryopreservation, recent methods were used, namely vitrification (dehydration in PVS2 solution, consisted of 30% (w/v) glycerol, 15% (w/v) ethylene glycol, and 15% (w/v) dimethyl sulfoxide, prepared in modified Vacin and Went liquid medium), encapsulation-dehydration (encapsulation in calcium alginate beads followed by airdrying in a laminar air-flow cabinet), encapsulation-vitrification (encapsulation in calcium alginate beads followed by dehydration in PVS2 solution), droplet-vitrification (fast freezing from small drops of PVS2 solution on aluminium strip), and cryo-plate (a combination of encapsulation and droplet on very fast freezing aluminium plate) dehydrated with silica gel and drying beads. Application of these methods in seeds was successful in Dendrobium chrysotoxum (99% vitrification), D. cruentum (32% vitrification; 58% D cryo-plate), D. draconis (95% vitrification), D. hercoglossum (80% encapsulation-vitrification), Doritis pulcherrima (62% vitrification), Paphiopedilum exul (30% encapsulation-vitrification; 14% encapsulation-dehydration), Rhynchostylis coelestis (85% vitrification), Vanda coerulea (67% vitrification) as well as in protocorms of Acampe rigida (17% V cryo-plate; 74% D cryo-plate), Arundina graminifolia (76% and 74% cryo-plate dehydrated with drying beads and silica gel, respectively; 33% droplet-vitrification; 64% encapsulation-dehydration with drying beads or silica gel), Dendrobium cariniferum (15%, encapsulation-vitrification), D. cruentum (33% vitrification; 27% encapsulation-dehydration), Grammaytophyllum speciosum (14% encapsulation-vitrification), Rhynchostylis gigantea (19% vitrification), Seidenfadenia mitrata (67% vitrification), Vanda coerulea (40% encapsulation-dehydration), and in pollinia of Dendrobium signatum (56% and 50% V cryo-plate and D cryo-plate, respectively). Cryopreserved seeds, protocorms, and pollinia were able to develop into normal seedlings/plantlets. These methods appear to be promising techniques for cryopreservation of some Thai orchid species.